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Fibroblast Growth Factor 1, Human, Active FGF-1

Product#: FNK-03-003

$261.32

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Fibroblast Growth Factor 1, Human, Active <FGF-1>

Cat. No. FNK-03-003
Store at -20°C
Size 50µg

Description

FGF-1 (acidic FGF) is a member of the fibroblast growth factor (FGF) family, which binds heparin. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion (ref.).

Fig.1 SDS-PAGE of human FGF-1

Applications

Use as a supplement in serum-free or reduced serum media for culture of mammalian cells
Studies of the human FGF-1 receptor, transmembrane signaling and protein phosphorylation
Western blotting control for anti-EGF-1 antibodies
Widely used in cosmetics, such as whitening, anti-wrinkle, anti-aging, etc.

Specification

Product

Full length recombinant mature FGF-1 (15.8 kDa, 140 amino acids) expressed in E. coli.

Activity

The ED50 as determined by a cell proliferation assay using MTS assay kit (Cell Titer 96, Promega) with Balb/c3T3 cells was < 10 pg/ml, corresponding to a specific activity of < 1 x 108
units/mg.

Purity

>98% as determined by SDS-PAGE (CBB staining)

Form

2.0 mg/ml in PBS (10mM Na-phosphate, 150mM NaCl) pH7.2, 50% glycerol, filter-sterilized

Storage

-20℃ (for long-term storage, -70℃)

Data Link

Gene ID: 2246,
Gene Sequence: BC032697,
Amino Acid Sequence: AAH32697

Reference

Zakrzewska M et al (2008) “ FGF-1: from biology through engineering to potential medical applications.” Review Crit Rev Clin Lab Sci 45: 91-135 PMID: 18293181